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CD44 Antibody

CAT: 0800-R31746Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R31746Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Cluster of differentiation 44 is also known as LHR or MC56. CD44 is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs) . This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for its gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunofluorescence (FFPE) : 2-4 µg/mL
Host
Rabbit
Reactivity
Human, Rat
Immunogen
An amino acid sequence from the N-terminus of human CD44 (RFAGVFHVEKNGRYSISRTEAADLCKAF) was used as the immunogen for this CD44 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IF, IHC-P
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Reconstitution
After reconstitution, the CD44 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This CD44 antibody is available for research use only.
Storage Conditions
After reconstitution, the CD44 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the CD44 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Cell surface, cytoplasmic
Image Legend
Immunofluorescent staining of FFPE human tonsil with CD44 antibody (green) at 1ug/ml and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.

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