Rab3A Antibody

• Description:

  Ras-related protein Rab-3A is a protein that in humans is encoded by the RAB3A gene. Synapsin I stimulated the Rab3A cycle by increasing GTP binding, GTPase activity, and Rab3A recruitment to the synaptic vesicle membrane. Conversely, Rab3A inhibited Synapsin I binding to actin and Synapsin I-induced synaptic vesicle clustering. It has been found that the protein is necessary for both forms of synaptic plasticity, and it is also involved in calcium exocytosis in neurons. In addition to that, Rab3A has been found to regulate a late step in synaptic vesicle fusion.

• Specifications:

  Western blot: 0.5-1 µg/mL

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Amino acids 192-220 (DTADPAVTGAKQGPQLSDQQVPPHQDCAC-human) were used as the immunogen for this Rab3A antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

• Reconstitution:

  After reconstitution, the Rab3A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This Rab3A antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the Rab3A antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  The stated application concentrations are suggested starting amounts. Titration of the Rab3A antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of Rab3A antibody and Lane 1: rat brain; 2: mouse brain lysate