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RAB5A Antibody

CAT: 0800-RQ4897Size: 100 µgDry Ice: NoHazardous: No
CAT#:0800-RQ4897Size:100 µg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
RAB5A (Ras-associated protein RAB5A), also called RAB5, is a protein that in humans is encoded by the RAB5A gene. RAB5 is a rate-limiting component of the machinery regulating the kinetics of membrane traffic in the early endocytic pathway. The RAB5A gene is mapped on 3p24.3. RAB5 is indispensable for a form of receptor tyrosine kinase-induced actin remodeling called circular ruffling. It signals to the actin cytoskeleton through RNTRE, a RAB5-specific GTPase-activating protein (GAP) .RAB5 activity on phagosome membranes began to increase on disassembly of the actin coat encapsulating phagosomes. In addition, RAB5 activation is either continuous or repetitive for up to 10 minutes, but it ends before the collapse of engulfed apoptotic cells. Expression of a dominant-negative mutant of RAB5 delayed this collapse of apoptotic thymocytes, showing a role for RAB5 in phagosome maturation.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Flow Cytometry: 1-3ug/10^6 cells, Immunofluorescence: 2-4 µg/mL
UniProt
P20339
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
Amino acids KKLPKNEPQNPGANSARGRGVDLTEPTQPTRNQCCSN from the human protein were used as the immunogen for the RAB5A antibody.
Clonality
Monoclonal
Isotype
IgG2b
Clone
3E9.
Applications
WB, FACS, IF
Purity
Purified
Format
Purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the RAB5A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This RAB5A antibody is available for research use only.
Storage Conditions
After reconstitution, the RAB5A antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the RAB5A antibody should be determined by the researcher.
Image Legend
Western blot testing of human 1) HeLa, 2) placenta, 3) A549, 4) A375, 5) SW620, 6) U-2 OS, 7) HEK293, 8) U-87 MG, 9) rat brain, 10) mouse brain and 11) mouse Neuro-2a lysate with RAB5A antibody. Predicted molecular weight ~25 kDa.
Product Datasheet
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