CD86 Antibody

• Description:

  Cluster of Differentiation 86 (also known as B7-2) is a protein expressed on antigen-presenting cells that provides costimulatory signals necessary for T cell activation and survival. The CD86 gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. The antigen presentation coactivators B7-1 and B7-2/CD86, which are important in other immune-mediated thyroid diseases, are important for lymphocytic infiltration and the immune response against thyroid carcinoma.

• Specifications:

  Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  Human partial recombinant protein (AA 24-329) was used as the immunogen for this CD86 antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, IHC-P

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose

• Reconstitution:

  The lyophilized CD86 antibody can be stored at 4oC to -20oC. After reconstitution, aliquot and store at -20oC. Avoid repeated freeze/thaws.

• Limitations:

  This CD86 antibody is available for research use only.

• Storage Conditions:

  The lyophilized CD86 antibody can be stored at 4°C to -20°C. After reconstitution, aliquot and store at -20°C. Avoid repeated freeze/thaws.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Titration of the CD86 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of mouse 1) spleen, 2) thymus, 3) J774A.1 and 4) ANA-1 cell lysate with CD86 antibody. Predicted molecular weight ~38 kDa (unmodified), 45-70 kDa (glycosylated) .