GAD67 Antibody

• Description:

  Glutamate decarboxylase 1 (GAD67), also known as GAD1, is a human gene. This gene encodes one of several forms of glutamic acid decarboxylase identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this GAD67 has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. It may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form.

• Specifications:

  Western blot: 0.5-1 µg/mL

• Host:

  Rabbit

• Reactivity:

  Human, Rat

• Immunogen:

  Human partial recombinant protein (AA 14-122) was used as the immunogen for this GAD67 antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

• Reconstitution:

  After reconstitution, the GAD67 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This GAD67 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the GAD67 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  The stated application concentrations are suggested starting amounts. Titration of the GAD67 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of GAD67 antibody and Lane 1: rat testis; 2: mouse brain; 3: human MCF7 lysate. Predicted/observed molecular weight ~67 kDa