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GAD65 Antibody

CAT: 0800-R30474Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R30474Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Glutamate decarboxylase or glutamic acid decarboxylase is an enzyme that catalyzes the decarboxylation of glutamate to GABA and CO2. GAD65 is the isoform with molecular weight of 65 kDa. Karlsen et al. (1991) mapped the GAD2 gene to chromosome 10p13-p11.2 by in situ hybridization. Bu et al. (1992) isolated a cDNA corresponding to the GAD2 gene from a human brain cDNA library. The deduced 585-amino acid protein has a molecular mass of 65 kD. GAD65 is a target of auto-antibodies in people who later develop type 1 diabetes mellitus or latent auto-immune diabetes.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL
UniProt
Q05329
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
An amino acid sequence from the N-terminus of the human protein (ENPGTARAWCQVAQKFT) was used as the immunogen for this GAD65 antibody (100% homologous in human, mouse and rat) .
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
After reconstitution, the GAD65 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This GAD65 antibody is available for research use only.
Storage Conditions
After reconstitution, the GAD65 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting points. Titration of the GAD65 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Cytoplasmic, membranous
Image Legend
Western blot testing of GAD65 antibody and rat brain tissue lysate. Predicted molecular weight ~65 kDa.

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