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GAD65 Antibody / GAD2

CAT: 0800-V7506SAF-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V7506SAF-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This mAb recognizes a protein of 65kDa, which is identified as glutamic acid decarboxylase 2 (GDA2) . It is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. There are two forms of glutamic acid decarboxylases (GADs) that are found in the brain: GAD2 (also known as GAD65) and GAD1 (also known as GAD67) . GAD1 and GAD2 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate-limiting step in the production of GABA (gamma-aminobutyric acid) from L-glutamic acid. Although both GAD s are found in the brain, GAD2 localizes to synaptic vesicle membranes in nerve terminals, while GAD1 is distributed throughout the cell. A pathogenic role for GAD2 is identified in the human pancreas since it has been identified as an autoantibody and an auto-reactive T cell target in insulin-dependent diabetes.
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 0.1-0.2 µg/mL for 30 min at RT
UniProt
Q05329
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 6-99 from the human protein were used as the immunogen for this GAD65 antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
GAD2/2362
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Store the GAD65 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This GAD65 antibody is available for research use only.
Storage Conditions
Store the GAD65 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Location
Cytoplasmic
Image Legend
IHC testing of FFPE human pancreas with GAD65 antibody (clone GAD2/2362) . Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min and allow to cool prior to testing.

UniProtKB · Q05329

Glutamate decarboxylase 2

DCE2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q05329
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
GAD2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
4.1.1.15
Processing
—
Secondary accessions
Q9UD87
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Cellular component

Cell membraneCell projectionCytoplasmCytoplasmic vesicleGolgi apparatusMembraneSynapse

Molecular function

DecarboxylaseLyase

PTM

LipoproteinPalmitatePhosphoprotein

Biological process

Neurotransmitter biosynthesis

Ligand

Pyridoxal phosphate

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