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SIRT1 Antibody

CAT: 0800-R31578Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R31578Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Sirtuin 1, also known as SIR2L1, is a protein that in humans is encoded by the SIRT1 gene. Sirtuin 1 is a member of the sirtuin family of proteins, homologs of the Sir2 gene in S. cerevisiae. Family members are characterized by a sirtuin core domain and grouped into four classes. Sirtuin 1 is downregulated in cells that have high insulin resistance and inducing its expression increases insulin sensitivity, suggesting the molecule is associated with improving insulin sensitivity. Furthermore, SIRT1 was shown to de-acetylate and affect the activity of both members of the PGC1-alpha/ERR-alpha complex, which are essential metabolic regulatory transcription factors.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL
UniProt
Q96EB6
Host
Rabbit
Reactivity
Human
Immunogen
Human partial recombinant protein (AA 112-311) was used as the immunogen for this SIRT1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IF
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Reconstitution
After reconstitution, the SIRT1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This SIRT1 antibody is available for research use only.
Storage Conditions
After reconstitution, the SIRT1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting points. Titration of the SIRT1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Cytoplasm, Mitochondrion and Nucleus
Image Legend
Immunofluorescent staining of FFPE human A431 cells with SIRT1 antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.

UniProtKB · Q96EB6

NAD-dependent protein deacetylase sirtuin-1

SIR1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q96EB6
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
SIRT1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
hSIRT1
EC number
2.3.1.286
Processing
—
Secondary accessions
Q2XNF6, Q5JVQ0, Q9GZR9, Q9Y6F0
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

AcetylationMethylationPhosphoproteinS-nitrosylationUbl conjugation

Coding sequence diversity

Alternative splicing

Biological process

ApoptosisBiological rhythmsDifferentiationHost-virus interactionMyogenesisTranscriptionTranscription regulation

Cellular component

CytoplasmMitochondrionNucleus

Molecular function

Developmental proteinTransferase

Ligand

Metal-bindingNADZinc

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