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Cyclin D3 Antibody

CAT: 0800-R31528Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R31528Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Cyclin D3 is a protein that in humans is encoded by the CCND3 gene. It is mapped to 6p21.1. The protein encoded by this gene belongs to a family of proteins whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin D3 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. In addition, overexpression of CCND3 upregulated the translational activity in HeLa cells in a dose-dependent manner.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL
UniProt
P30281
Host
Rabbit
Reactivity
Human, Rat
Immunogen
Human partial recombinant protein (AA 136-292) was used as the immunogen for this Cyclin D3 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Reconstitution
After reconstitution, the Cyclin D3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Cyclin D3 antibody is available for research use only.
Storage Conditions
After reconstitution, the Cyclin D3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the Cyclin D3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Nuclear
Image Legend
Western blot testing of Cyclin D3 antibody and Lane 1: rat testis; 2: (r) thymus; 3: (r) lung; 4: (r) ovary; 5: human Jurkat; 6: (h) A549; 7: (h) MCF-7; 8: (h) HeLa lysate. Predicted molecular weight ~33 kDa.

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