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Cyclin B1 Antibody

CAT: 0800-V2946SAF-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V2946SAF-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
It recognizes a protein of 55-62kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.
CAS Number
9007-83-4
Specifications
Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P14635
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human full-length protein was used as the immunogen for the Cyclin B1 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
CCNB1/1098
Applications
IHC-P, IF
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Store the Cyclin B1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Cyclin B1 antibody is available for research use only.
Storage Conditions
Store the Cyclin B1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Location
Cytoplasmic & nuclear
Image Legend
IHC: Formalin-fixed, paraffin-embedded human endometrial carcinoma stained with Cyclin B1 antibody (CCNB1/1098) .

UniProtKB · P14635

G2/mitotic-specific cyclin-B1

CCNB1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P14635
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
CCNB1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
A8K066, Q5TZP9
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

AcetylationPhosphoproteinUbl conjugation

Coding sequence diversity

Alternative splicing

Biological process

Cell cycleCell divisionMitosis

Molecular function

Cyclin

Cellular component

CytoplasmCytoskeletonNucleus

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