Cyclin T1 Antibody

CAT: 0800-R32475Size: 100 µgDry Ice: NoHazardous: No

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CAT#:0800-R32475Size:100 µg

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Description

Cyclin-T1 is a protein that in humans is encoded by the CCNT1 gene. This gene encodes a member of the highly conserved cyclin C subfamily. The encoded protein tightly associates with cyclin-dependent kinase 9, and is a major subunit of positive transcription elongation factor b (p-TEFb) . In humans, there are multiple forms of positive transcription elongation factor b, which may include one of several different cyclins along with cyclin-dependent kinase 9. The complex containing the encoded cyclin and cyclin-dependent kinase 9 acts as a cofactor of human immunodeficiency virus type 1 (HIV-1) Tat protein, and is both necessary and sufficient for full activation of viral transcription. This cyclin and its kinase partner are also involved in triggering transcript elongation through phosphorylation of the carboxy-terminal domain of the largest RNA polymerase II subunit. Overexpression of this gene is implicated in tumor growth. Alternative splicing results in multiple transcript variants.

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunofluorescence/Immunocytochemistry: 2-4 µg/mL, Flow cytometry: 1-3ug/10^6 cells

UniProt

O60563

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids QKQNSKSVPSAKVSLKEYRAKHAEELAAQKRQLENM from the human protein were used as the immunogen for the Cyclin T1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF/ICC, FACS

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide

Reconstitution

Prior to reconstitution, store at 4oC. After reconstitution, the Cyclin T1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Cyclin T1 antibody is available for research use only.

Storage Conditions

Prior to reconstitution, store at 4°C. After reconstitution, the Cyclin T1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Cyclin T1 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

Western blot testing of 1) rat kidney, 2) mouse spleen and 3) human Jurkat lysate with Cyclin T1 antibody at 0.5ug/ml. Predicted/observed molecular weight ~81 kDa.

UniProtKB · O60563

Cyclin-T1

CCNT1_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: O60563
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: CCNT1
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: CycT1, Cyclin-T
EC number: —
Processing: —
Secondary accessions: A9XU13, E7EX76, O60581

Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationADP-ribosylationIsopeptide bondPhosphoproteinUbl conjugation

Coding sequence diversity

Alternative splicing

Biological process

Cell cycleCell divisionHost-virus interactionTranscriptionTranscription regulation

Domain

Coiled coil

Molecular function

Cyclin

Cellular component

Nucleus

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Alternative Products

Catalog	Name
MBS9435956-01	Cyclin A1+ Cyclin A2 Antibody
abx134059-01	Cyclin A1 (Cyclin A1) Antibody
abx133138-01	Cyclin H (Cyclin H) Antibody
abx225081-01	Cyclin D2 (Cyclin D2) Antibody
abx007561-01	Cyclin E1 (Cyclin E1) Antibody
abx225079-01	Cyclin A2 (Cyclin A2) Antibody
10R-7848	Cyclin A antibody