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IL-18 Antibody

CAT: 0800-R31447Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R31447Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Interleukin-18, also called Interferon-gamma-inducing factor (IGIF), augments natural killer (NK) activity in spleen cells. The gene encodes a precursor protein of 192 amino acids and a mature protein of 157 amino acids. IL-18 is a cytokine that modulates both T helper type 1 (Th1) and Th2 responses. It is a potent proinflammatory cytokine with potential atherogenic properties. It is highly expressed in the atherosclerotic plaques compared with control normal arteries and is localized mainly in plaque macrophages.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells, Immunohistochemistry (FFPE) : 2-5 µg/mL
Host
Rabbit
Reactivity
Rat, Mouse
Immunogen
Rat partial recombinant protein (AA 37-194) was used as the immunogen for this IL-18 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, FACS, IHC-P
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.005% sodium azide
Reconstitution
After reconstitution, the IL-18 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This IL-18 antibody is available for research use only.
Storage Conditions
After reconstitution, the IL-18 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the IL-18 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
IHC staining of FFPE rat spleen tissue with IL-18 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

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