Interleukin 18 Antibody / IL-18

• Description:

  Four structurally related IL-1 receptor ligands have been described. These include three agonists designated IL-1a, IL-1b and IL-1g/IL-18 and a specific receptor antagonist, IL-1Ra. IL-1a and IL-1b play critical roles in the regulation of the immune response and inflammation, serving as activators of T and B lymphocytes and NK (natural killer) cells. IL-18 (also referred to as IL-1g) has been shown to augment the secretion of IFN-g from T lymphocytes and increase NK cell activity in spleen cells. IL-18 exhibits 19% and 12% identity with IL-1a and IL-1b respectively over the 12 beta-strands of the beta-trefoil fold domain, which is a signature feature of the IL-1 family. The unusual leader sequence of IL-18 may be analogous to the IL-1b pro-domain which must be cleaved by the serine protease ICE for optimal secretion and biological activity. Originally described as IGIF (IFN-g-inducing factor), IL-18 is induced by mouse liver subsequent to challenge with lipopolysaccharide (LPS) .

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 2-4 µg/mL

• UniProt:

  Q14116

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A recombinant fragment (within amino acids 1-193) of human IL-18 protein was used as the immunogen for the Interleukin 18 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  IL18/4623

• Applications:

  IHC-P, WB

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Aliquot the Interleukin 18 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This Interleukin 18 antibody is available for research use only.

• Storage Conditions:

  Aliquot the Interleukin 18 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the Interleukin 18 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Secreted

• Image Legend:

  Western blot testing of human A549 cell lysate with Interleukin 18 antibody. Predicted molecular weight ~22 kDa.