Caspase-14 Antibody

CAT: 0800-R31216Size: 100 µgDry Ice: NoHazardous: No

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CAT#:0800-R31216Size:100 µg

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Description

Caspase 14 is an enzyme that in humans is encoded by the CASP14 gene. CASP14 belongs the evolutionarily conserved caspase family. CASP14 was assigned to chromosome 19p13.1. Using Northern blot and semiquantitative RT-PCR analyses, it was showed that CASP14 was transcriptionally upregulated during maintenance of confluent cultures of human epidermal keratinocytes. The expression and processing of this caspase may be involved in keratinocyte terminal differentiation, which is important for the formation of the skin barrier. Caspase 14 seems to play a role in keratinocyte differentiation and cornification and probably regulates maturation of the epidermis by proteolytically processing filaggrin.

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL

UniProt

P31944

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids 223-242 (KARKTNPEIQSTLRKRLYLQ-human) were used as the immunogen for this Caspase-14 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the Caspase-14 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Caspase-14 antibody is available for research use only.

Storage Conditions

After reconstitution, the Caspase-14 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Caspase-14 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of Caspase-14 antibody; Lane 1: rat brain; 2: rat liver; 3: rat spleen; 4: human A431; 5: mouse NIH3T3

UniProtKB · P31944

Caspase-14

CASPE_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P31944
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: CASP14
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: CASP-14
EC number: 3.4.22.-
Processing: Precursor
Secondary accessions: O95823, Q3SYC9

Protein keywords

Cellular component

CytoplasmNucleus

Biological process

Differentiation

Technical term

Direct protein sequencingProteomics identificationReference proteome

Molecular function

HydrolaseProteaseThiol protease

Disease

Ichthyosis

PTM

Zymogen

Product Datasheet

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Alternative Products

Catalog	Name
70R-13452	Caspase 14 antibody
3011-30T	Caspase-14 Antibody
309278-01	Caspase-14 (CASP14, Caspase-14, CASP-14)