CD14 Antibody

• Description :

  CD14 is a surface protein preferentially expressed on monocytes/macrophages. It binds lipopolysaccharide binding protein and recently has been shown to bind apoptotic cells.

• Specifications :

  Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50, Immunofluorescence: 1:10-1:50, Flow Cytometry: 1:10-1:50

• UniProt :

  P08571

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  A portion of amino acids 54-83 from the human protein was used as the immunogen for this CD14 antibody.

• Clonality :

  Polyclonal

• Isotype :

  Ig

• Applications :

  WB, IHC, IF, FACS, ELISA

• Purity :

  Purified

• Format :

  Purified

• Buffer :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution :

  Aliquot the CD14 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations :

  This CD14 antibody is available for research use only.

• Storage Conditions :

  Aliquot the CD14 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes :

  Titration of the CD14 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number :

  9007-83-4

• Location :

  Cell surface, Secreted, Cytoplasmic (Golgi)

• Image Legend :

  Confocal immunofluorescent analysis of CD14 antibody with A549 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) . DAPI was used as a nuclear counterstain (blue) .