CD14 Antibody

• Description :

  Recognizes a protein of 55kDa, identified as CD14 (also known lipopolysaccharide receptor) . CD14 is expressed strongly on monocytes and macrophage and weakly on the surface of neutrophils. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP) . Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation.

• Specifications :

  Immunohistochemistry (FFPE) : 1-2 µg/mL

• UniProt :

  P08571

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  A recombinant fragment of human CD14 protein (within amino acids 1-200) was used as the immunogen for the CD14 antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG2 κ

• Clone :

  LPSR/7738

• Applications :

  IHC-P

• Purity :

  Protein A/G affinity

• Format :

  Purified

• Buffer :

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution :

  Aliquot the CD14 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations :

  This CD14 antibody is available for research use only.

• Storage Conditions :

  Aliquot the CD14 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation :

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes :

  Optimal dilution of the CD14 antibody should be determined by the researcher.

• CAS Number :

  9007-83-4

• Location :

  Cell membrane

• Image Legend :

  IHC staining of FFPE human tonsil tissue with CD14 antibody (clone LPSR/7738) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.