CD14 Antibody

CAT: 0800-V5447SAF-100UGSize: 100 µgDry Ice: NoHazardous: No
CAT#:0800-V5447SAF-100UGSize:100 µg
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Description
Recognizes a protein of 55kDa, identified as CD14 (also known lipopolysaccharide receptor) . CD14 is expressed strongly on monocytes and macrophage and weakly on the surface of neutrophils. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP) . Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation.
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P08571
Host
Mouse
Reactivity
Human
Immunogen
A recombinant fragment of human CD14 protein (within amino acids 1-200) was used as the immunogen for the CD14 antibody.
Clonality
Monoclonal
Isotype
IgG2 κ
Clone
LPSR/7738
Applications
IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Aliquot the CD14 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This CD14 antibody is available for research use only.
Storage Conditions
Aliquot the CD14 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Optimal dilution of the CD14 antibody should be determined by the researcher.
Location
Cell membrane
Image Legend
IHC staining of FFPE human tonsil tissue with CD14 antibody (clone LPSR/7738) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

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