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Cdk4 Antibody

CAT: 0800-R30500Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R30500Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Cyclin-dependent kinase-4 (CDK4) is a protein-serine kinase involved in the cell cycle. Human cell division is regulated primarily at the G1-to-S or the G2-to-M boundaries within the cell cycle. The complexes formed by CDK4 and the D-type cyclins are involved in the control of cell proliferation during the G1 phase. CDK4 is inhibited by p16, also known as cyclin-dependent kinase inhibitor-2. CDK4 is mapped to 12q14. CDK4 expression and activity are required for cytokine responsiveness in T cells.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
P30285
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
An amino acid sequence from the C-terminus of mouse Cdk4 (RISAFRALQHSYLHKEESDAE) was used as the immunogen for this Cdk4 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IF, FACS
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
After reconstitution, the Cdk4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Cdk4 antibody is available for research use only.
Storage Conditions
After reconstitution, the Cdk4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the Cdk4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Nuclear, cytoplasmic
Image Legend
Immunofluorescent staining of FFPE human MCF7 cells with Cdk4 antibody (red) and Alpha Tubulin mAb (green) . HIER: steam section in pH6 citrate buffer for 20 min.
Product Datasheet
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