Caspase-3 Antibody

CAT: 0800-R30358Size: 100 µgDry Ice: NoHazardous: No

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CAT#:0800-R30358Size:100 µg

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Description

Caspase-3, also known as YAMA, CPP32 or APOPAIN, is a member of the cysteine-aspartic acid protease family known to interact with family members -8 and -9. It is encoded by the CASP3 gene. Tiso et al. (1996) used radiation hybrid mapping to localize the gene to human chromosome 4q33-q35.1. Fernandes-Alnemri et al. (1994) found that overexpression of CASP3 in insect cells induced apoptosis. Coexpression of the two CASP3 subunits in insect cells also resulted in apoptosis. Tewari et al. (1995) showed that purified human CASP1 cleaved the CASP3 proenzyme into a proteolytically active form and that activated Caspase-3 cleaved PARP into the 85-kD apoptotic form.

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, Immunocytochemistry: 0.5-1 µg/mL

UniProt

P42574

Host

Rabbit

Reactivity

Human

Immunogen

An amino acid sequence from the C-terminus of the human protein (CAMLKQYADKLEFMHIL) was used as the immunogen for this Caspase-3 antibody. This amino acid sequence is from the small subunit.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, ICC

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

Reconstitution

After reconstitution, the Caspase-3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Caspase-3 antibody is available for research use only.

Storage Conditions

After reconstitution, the Caspase-3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Caspase-3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of Caspase-3 antibody and Lane 1: HeLa; 2: SMMC-7721 cell lysate

UniProtKB · P42574

Caspase-3

CASP3_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P42574
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: CASP3
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: CASP-3
EC number: 3.4.22.56
Processing: Precursor
Secondary accessions: A8K5M2, D3DP53, Q96AN1, Q96KP2

Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationPhosphoproteinS-nitrosylationUbl conjugationZymogen

Biological process

Apoptosis

Cellular component

Cytoplasm

Molecular function

HydrolaseProteaseThiol protease

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Alternative Products

Catalog	Name
GTR18074854-01	Caspase-3 Antibody
GTR18305414	Caspase 3 Antibody