Products for Research Use Only
RegisterLogin

Alpha-Adaptin Antibody / AP2A1

CAT: 0800-R30093Size: 100 µgDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0800-R30093Size:100 µg
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
Alpha Adaptin, also known as adaptor related protein complex, alpha 1 subunit. The alpha adaptins are exclusively found in the endocytic coated vesicles. Huntingtin-interacting protein 1 (HIP1) is enriched in membrane-containing cell fractions and has been implicated in vesicle trafficking. Waelter et al. (2001) identified 3 HIP1-associated proteins, clathrin heavy chain (CLTC) and alpha-adaptin A and C. Coat proteins of approximately 100-kD (adaptins) are components of the adaptor complexes which link clathrin to receptors in coated vesicles. The alpha-adaptins separate into two bands on SDS gels.
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL
UniProt
O95782
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
AP-2 adaptor polypeptides from bovine brain was used as the immunogen for this Alpha-Adaptin antibody.
Clonality
Monoclonal
Isotype
IgG2a
Clone
100/2
Applications
WB
Purity
Ascites
Format
Ascites
Buffer
0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Reconstitution
After reconstitution, the alpha-Adaptin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Alpha-Adaptin antibody is available for research use only.
Storage Conditions
After reconstitution, the Alpha-Adaptin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the Alpha-Adaptin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of 1) human SH-SY5Y, 2) human U-87 MG, 3) human MCF7, 4) human 293T, 5) rat brain, 6) rat C6 and 7) mouse brain tissue lysate with Alpha-Adaptin antibody. Predicted molecular weight ~108 kDa.

UniProtKB · O95782

AP-2 complex subunit alpha-1

AP2A1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
O95782
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
AP2A1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
Q96CI7, Q96PP6, Q96PP7, Q9H070
Protein keywords

Coding sequence diversity

Alternative splicing

Cellular component

Cell membraneCoated pitMembrane

Biological process

EndocytosisProtein transportTransport

PTM

Phosphoprotein

Technical term

Proteomics identificationReference proteome

Related Products

CatalogName
RQ6543AP2A1 Antibody / Alpha Adaptin
RQ4811AP2A1 Antibody / Alpha 1 adaptin
R33860-100UGAP2A1 Antibody
R33860BTNAP2A1 Antibody (Biotin Conjugate)
RQ6600AP2A2 Antibody / AP-2 complex subunit alpha-2
V5187-100UGER alpha Antibody / Estrogen Receptor alpha
V5170-20UGHCG-alpha Antibody / Luteinizing Hormone alpha
V7015SAF-100UGHCG alpha Antibody / Luteinizing Hormone alpha
V5170-100UGHCG-alpha Antibody / Luteinizing Hormone alpha
V5170SAF-100UGHCG-alpha Antibody / Luteinizing Hormone alpha

Alternative Products

CatalogName
MBS1755679-01Anti-alpha Adaptin/AP2A1 Antibody Picoband
GTR17694518Alpha Adaptin/AP2A1 Rabbit Polyclonal Antibody
A08806-1Anti-alpha Adaptin/AP2A1 Antibody Picoband®
GTR17689349Alpha-Adaptin AP2A1 Mouse Monoclonal Antibody
GTR18747157Alpha Adaptin/AP2A1 Rabbit Polyclonal Antibody (Fluoro550)
GTR18548438Alpha Adaptin/AP2A1 Rabbit Polyclonal Antibody (HRP)
GTR18548441Alpha Adaptin/AP2A1 Rabbit Polyclonal Antibody (Biotin)
GTR18548436Alpha Adaptin/AP2A1 Rabbit Polyclonal Antibody (PE)
GTR18747146Alpha Adaptin/AP2A1 Rabbit Polyclonal Antibody (Fluoro594)
GTR18747135Alpha Adaptin/AP2A1 Rabbit Polyclonal Antibody (Fluoro647)

Popular Products