Alpha-Adaptin Antibody / AP2A1

• Description:

  Alpha Adaptin, also known as adaptor related protein complex, alpha 1 subunit. The alpha adaptins are exclusively found in the endocytic coated vesicles. Huntingtin-interacting protein 1 (HIP1) is enriched in membrane-containing cell fractions and has been implicated in vesicle trafficking. Waelter et al. (2001) identified 3 HIP1-associated proteins, clathrin heavy chain (CLTC) and alpha-adaptin A and C. Coat proteins of approximately 100-kD (adaptins) are components of the adaptor complexes which link clathrin to receptors in coated vesicles. The alpha-adaptins separate into two bands on SDS gels.

• Specifications:

  Western blot: 1-2 µg/mL

• UniProt:

  O95782

• Host:

  Mouse

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  AP-2 adaptor polypeptides from bovine brain was used as the immunogen for this Alpha-Adaptin antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2a

• Clone:

  100/2

• Applications:

  WB

• Purity:

  Ascites

• Format:

  Ascites

• Buffer:

  0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS

• Reconstitution:

  After reconstitution, the alpha-Adaptin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This Alpha-Adaptin antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the Alpha-Adaptin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS

• Applications Notes:

  The stated application concentrations are suggested starting amounts. Titration of the Alpha-Adaptin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of 1) human SH-SY5Y, 2) human U-87 MG, 3) human MCF7, 4) human 293T, 5) rat brain, 6) rat C6 and 7) mouse brain tissue lysate with Alpha-Adaptin antibody. Predicted molecular weight ~108 kDa.