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AP2A1 Antibody / Alpha Adaptin

CAT: 0800-RQ6543Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ6543Size:100 µg
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Description
AP-2 complex subunit alpha-1 is a protein that in humans is encoded by the AP2A1 gene. This gene encodes the alpha 1 adaptin subunit of the adaptor protein 2 (AP-2) complex found in clathrin coated vesicles. The AP-2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma) . The complex is part of the protein coat on the cytoplasmic face of coated vesicles which links clathrin to receptors in vesicles. Alternative splicing of this gene results in two transcript variants encoding two different isoforms. A third transcript variant has been described, but its full length nature has not been determined.
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL
UniProt
O95782
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
An E. coli-derived human protein (amino acids S785-F977) was used as the immunogen for the AP2A1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, FACS, Direct ELISA
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the AP2A1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This AP2A1 antibody is available for research use only.
Storage Conditions
After reconstitution, the AP2A1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the AP2A1 antibody should be determined by the researcher.
Image Legend
Flow cytometry testing of human Raji cells with AP2A1 antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= AP2A1 antibody.

UniProtKB · O95782

AP-2 complex subunit alpha-1

AP2A1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
O95782
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
AP2A1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
Q96CI7, Q96PP6, Q96PP7, Q9H070
Protein keywords

Coding sequence diversity

Alternative splicing

Cellular component

Cell membraneCoated pitMembrane

Biological process

EndocytosisProtein transportTransport

PTM

Phosphoprotein

Technical term

Proteomics identificationReference proteome
Product Datasheet
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