Bcl-2 Antibody

CAT: 0800-R30004Size: 100 µgDry Ice: NoHazardous: No
CAT#:0800-R30004Size:100 µg
Selected
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product image 1
1 / 1
Description
Immunoreactive BCL2 protein was detected in the neoplastic cells of almost all follicular lymphomas whereas no BCL2 protein was detected in follicles affected by nonneoplastic processes or in normal lymphoid tissue. Every tumor with molecular-genetic evidence of t (14;18) translocation expressed detectable levels of the protein, regardless of whether the breakpoint was located in or at a distance from the gene. Overexpression blocks the apoptotic death of a pro-B-lymphocyte cell line.
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, IHC (Frozen) : 0.5-1 µg/mL in formalin/acetone fixed tissues, Immunocytochemistry: Suitable
Host
Mouse
Reactivity
Human
Immunogen
Amino acids 41-54 of the human protein were used as the immunogen for this Bcl-2 antibody.
Clonality
Monoclonal
Isotype
IgG1
Clone
Bcl-2-100
Applications
WB, IHC-P, IHC-F, ICC
Purity
Ascites
Format
Ascites
Buffer
0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Reconstitution
After reconstitution, the Bcl-2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Bcl-2 antibody is available for research use only.
Storage Conditions
After reconstitution, the Bcl-2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS
Applications Notes
The stated application concentrations are suggested starting points. Titration of the Bcl-2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Cytoplasmic
Image Legend
Western blot testing of Bcl-2 antibody and rat tissue: 1. heart; 2. spleen; 3. small intestine; 4: liver. Predicted molecular weight ~26 kDa.

Popular Products