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RFC5 Antibody

CAT: 0800-F54778-0.08MLSize: 0.08 mLDry Ice: NoHazardous: No
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CAT#:0800-F54778-0.08MLSize:0.08 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) . RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. RFC5 is the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system.
CAS Number
9007-83-4
Specifications
Flow cytometry: 1:25 (1x10e6 cells), Western blot: 1:500-1:2000
UniProt
P40937
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 170-200 from the human protein was used as the immunogen for the RFC5 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
FACS, WB
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the RFC5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This RFC5 antibody is available for research use only.
Storage Conditions
Aliquot the RFC5 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
The stated application concentrations are suggested starting points. Titration of the RFC5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with RFC5 antibody.
Product Datasheet
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