RFC5 Antibody

• Description:

  The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) . RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. RFC5 is the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system.

• Specifications:

  Flow cytometry: 1:25 (1x10e6 cells), Western blot: 1:500-1:2000

• UniProt:

  P40937

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 170-200 from the human protein was used as the immunogen for the RFC5 antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  FACS, WB

• Purity:

  Purified

• Format:

  Purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the RFC5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This RFC5 antibody is available for research use only.

• Storage Conditions:

  Aliquot the RFC5 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  The stated application concentrations are suggested starting points. Titration of the RFC5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with RFC5 antibody.