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RFC3 Antibody / Replication factor C subunit 3

CAT: 0800-RQ7818Size: 100 µgDry Ice: NoHazardous: No
CAT#:0800-RQ7818Size:100 µg
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AVAILABILITY
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Replication factor C subunit 3, also called Activator 1 38 kDa subunit (A1 38 kDa subunit) and Replication factor C 38 kDa subunit (RF-C 38 kDa subunit or RFC38), is a protein that in humans is encoded by the RFC3 gene. The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) . RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kDa. This gene encodes the 38 kDa subunit. This subunit is essential for the interaction between the 140 kDa subunit and the core complex that consists of the 36, 37, and 40 kDa subunits. Alternatively spliced transcript variants encoding distinct isoforms have been described.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL
UniProt
P40938
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
E. coli-derived recombinant human protein (amino acids D6-E333) was used as the immunogen for the RFC3 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IF, FACS, Direct ELISA
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the RFC3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This RFC3 antibody is available for research use only.
Storage Conditions
After reconstitution, the RFC3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the RFC3 antibody should be determined by the researcher.
Location
Nuclear
Image Legend
Immunofluorescent staining of FFPE human PC-3 cells with RFC3 antibody (red) and Alpha Tubulin mAb (green) . HIER: steam section in pH6 citrate buffer for 20 min.
Product Datasheet
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