Products for Research Use Only
RegisterLogin

RFC1 Antibody / Replication factor C subunit 1

CAT: 0800-R30806Size: 100 µgDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0800-R30806Size:100 µg
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
Replication Factor C, also called Activator-1, is a multimeric primer-recognition protein consisting of five distinct subunits of 145, 40, 38, 37, and 36.5 kD. The human protein was purified from extracts of HeLa cells as a host factor essential for the in vitro replication of simian virus 40 (SV40) DNA. RFC, in the presence of ATP, assembles proliferating-cell nuclear antigen and DNA polymerase-delta or polymerase-epsilon on primed DNA templates. Tight interfacial coordination of the ATP analog ATP-gamma-S by RFC resulted in a spiral arrangement of the ATPase domains of the clamp loader above the PCNA ring.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
P35251
Host
Rabbit
Reactivity
Human
Immunogen
An amino acid sequence from the N-terminus of human RFC1 (NSSRKEDDFKQKQPSKKKR) was used as the immunogen for this RFC1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IF, FACS
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
After reconstitution, the RFC1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This RFC1 antibody is available for research use only.
Storage Conditions
After reconstitution, the RFC1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the RFC1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Nuclear
Image Legend
Immunofluorescent staining of FFPE human U-2 OS cells with RFC1 antibody (green) and Beta Tubulin mAb (red) . HIER: steam section in pH6 citrate buffer for 20 min.
Product Datasheet
Loading...

Related Products

CatalogName
RQ7817RFC2 Antibody / Replication factor C subunit 2
RQ7818RFC3 Antibody / Replication factor C subunit 3
RQ8160RFC4 Antibody / RCF37 / Replication factor C subunit 4
RQ6763CDT1 Antibody / Chromatin licensing and DNA replication factor 1
RQ7910CDT1 Antibody / DNA replication factor Cdt1
FY12426RTF2 Antibody / Replication termination factor 2
F54673-0.4MLSplicing factor 3B subunit 1 Antibody / SF3B1
F54673-0.08MLSplicing factor 3B subunit 1 Antibody / SF3B1
RQ7975EIF3C Antibody / Eukaryotic translation initiation factor 3 subunit C
RQ7633SF3a120 Antibody / Splicing factor 3A subunit 1 / SF3A1

Alternative Products

CatalogName
abx015116-01Replication Factor C Subunit 2 (RFC2) Antibody
abx141759-01Replication Factor C Subunit 4 (RFC4) Antibody
abx008772-01Replication Factor C Subunit 3 (RFC3) Antibody
abx001369-01Replication Factor C Subunit 1 (RFC1) Antibody
abx122998-01Replication Factor C Subunit 5 (RFC5) Antibody

Popular Products