RFC2 Antibody / Replication factor C subunit 2

CAT: 0800-RQ7817Size: 100 µgDry Ice: NoHazardous: No
CAT#:0800-RQ7817Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Replication factor C subunit 2, also called Activator 1 40 kDa subunit (A1 40 kDa subunit) and Replication factor C 40 kDa subunit (RF-C 40 kDa subunit or RFC40), is a protein that in humans is encoded by the RFC2 gene. This gene encodes a member of the activator 1 small subunits family. The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins, proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) . Replication factor C, also called activator 1, is a protein complex consisting of five distinct subunits. This gene encodes the 40 kD subunit, which has been shown to be responsible for binding ATP and may help promote cell survival. Disruption of this gene is associated with Williams syndrome. Alternatively spliced transcript variants encoding distinct isoforms have been described. A pseudogene of this gene has been defined on chromosome 2.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL
UniProt
P35250
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
E. coli-derived recombinant human protein (amino acids E40-K313) was used as the immunogen for the RFC2 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IF, FACS, Direct ELISA
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the RFC2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This RFC2 antibody is available for research use only.
Storage Conditions
After reconstitution, the RFC2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the RFC2 antibody should be determined by the researcher.
Location
Nuclear
Image Legend
Immunofluorescent staining of FFPE human PC-3 cells with RFC2 antibody (red) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.

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