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ATG4A Antibody

CAT: 0800-F54713-0.08MLSize: 0.08 mLDry Ice: NoHazardous: No
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CAT#:0800-F54713-0.08MLSize:0.08 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole) . APG4A is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II) . Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE) -conjugated form, and has the capacity for the binding to autophagosomes. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP.
CAS Number
9007-83-4
Specifications
Western blot: 1:500-1:2000, Immunohistochemistry (FFPE) : 1:25
UniProt
Q8WYN0
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
A portion of amino acids 363-392 from the human protein was used as the immunogen for the ATG4A antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, IHC-P
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the ATG4A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This ATG4A antibody is available for research use only.
Storage Conditions
Aliquot the ATG4A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
The stated application concentrations are suggested starting points. Titration of the ATG4A antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of mouse kidney tissue lysate with ATG4A antibody. Predicted molecular weight ~45 kDa, commonly observed between 45-60 kDa.

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