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ATG9A Antibody

CAT: 0800-F54719-0.4MLSize: 0.4 mLDry Ice: NoHazardous: No
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CAT#:0800-F54719-0.4MLSize:0.4 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole) . Apg9 plays a direct role in the formation of the cytoplasm to vacuole targeting and autophagic vesicles, possibly serving as a marker for a specialized compartment essential for these vesicle-mediated alternative targeting pathways.
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1:25, Western blot: 1:500-1:2000, Immunofluorescence: 1:25
UniProt
Q7Z3C6
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 717-746 from the human protein was used as the immunogen for the ATG9A antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
IHC-P, WB, IF
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the ATG9A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This ATG9A antibody is available for research use only.
Storage Conditions
Aliquot the ATG9A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
The stated application concentrations are suggested starting points. Titration of the ATG9A antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Cytoplasmic
Image Legend
Western blot testing of human 1) A375 and 2) HepG2 cell lysate with ATG9A antibody. Expected molecular weight: 94-110 kDa depending on glycosylation level.

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