ATG4B Antibody

CAT: 0800-F54715-0.08MLSize: 0.08 mLDry Ice: NoHazardous: No
CAT#:0800-F54715-0.08MLSize:0.08 mL
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole) . APG4 is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II) . Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE) -conjugated form, and has the capacity for the binding to autophagosomes.
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1:25, Western blot: 1:500-1:2000, Immunofluorescence: 1:25
UniProt
Q9Y4P1
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 358-390 from the human protein was used as the immunogen for the ATG4B antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
IHC-P, WB, IF
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the ATG4B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This ATG4B antibody is available for research use only.
Storage Conditions
Aliquot the ATG4B antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
The stated application concentrations are suggested starting points. Titration of the ATG4B antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Cytoplasmic
Image Legend
Western blot testing of human HeLa cell lysate with ATG4B antibody. Expected molecular weight: 37-52 kDa (multiple isoforms) .

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