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ATG9A Antibody

CAT: 0800-RQ5704Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ5704Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Autophagy-related protein 9A is a protein that in humans is encoded by the ATG9A gene. ATG9A is the only transmembrane ATG protein essential for autophagy. It plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS) . It has been reported that ATG9A expression is increased in oral squamous cell carcinoma and breast cancers. The inhibition of ATG9A can lead to an inhibition of cancer cell proliferation and invasion.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells, Immunofluorescence: 2-4 µg/mL, Direct ELISA: 0.1-0.5 µg/mL
UniProt
Q7Z3C6
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
Recombinant human protein (amino acids M1-D812) was used as the immunogen for the ATG9A antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, FACS, IF, Direct ELISA
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the ATG9A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This ATG9A antibody is available for research use only.
Storage Conditions
After reconstitution, the ATG9A antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the ATG9A antibody should be determined by the researcher.
Image Legend
Immunofluorescent staining of FFPE human A549 cells with ATG9A antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.

UniProtKB · Q7Z3C6

Autophagy-related protein 9A

ATG9A_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q7Z3C6
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ATG9A
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
Q3ZAQ6, Q6P0N7, Q7Z317, Q7Z320, Q8NDK6, Q8WU65, Q9BVL5, Q9H6L1, Q9HAG7
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

AcetylationGlycoproteinPhosphoproteinUbl conjugation

Coding sequence diversity

Alternative splicing

Biological process

AutophagyLipid transportTransport

Cellular component

Cytoplasmic vesicleEndoplasmic reticulumEndosomeGolgi apparatusMembraneMitochondrion

Domain

TransmembraneTransmembrane helix
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