CDK4 Antibody

CAT: 0800-F54049-0.1MLSize: 0.1 mLDry Ice: NoHazardous: No
CAT#:0800-F54049-0.1MLSize:0.1 mL
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Description
Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. [UniProt]
CAS Number
9007-83-4
Specifications
Western blot: 1:500
UniProt
P11802
Host
Mouse
Reactivity
Human
Immunogen
A human recombinant protein was used as the immunogen for this CDK4 antibody.
Clonality
Monoclonal
Isotype
LgG1
Clone
3D12-A7-F11
Applications
WB
Purity
Protein G affinity
Format
Purified
Buffer
Antibody in PBS with 50% Glycerol and 0.03% ProClin 300
Reconstitution
Store the CDK4 antibody at -20oC.
Limitations
This CDK4 antibody is available for research use only.
Storage Conditions
Store the CDK4 antibody at -20°C.
Formulation
In PBS with 50% glycerol and 0.03% ProClin 300
Applications Notes
The stated application concentrations are suggested starting points. Titration of the CDK4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of human 1) HeLa and 2) K562 cell lysates using CDK4 antibody at 1:500. Predicted molecular weight ~34 kDa.

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