CDK4 Antibody

• Description:

  Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. [UniProt]

• Specifications:

  Western blot: 1:500

• UniProt:

  P11802

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A human recombinant protein was used as the immunogen for this CDK4 antibody.

• Clonality:

  Monoclonal

• Isotype:

  LgG1

• Clone:

  3D12-A7-F11

• Applications:

  WB

• Purity:

  Protein G affinity

• Format:

  Purified

• Buffer:

  Antibody in PBS with 50% Glycerol and 0.03% ProClin 300

• Reconstitution:

  Store the CDK4 antibody at -20oC.

• Limitations:

  This CDK4 antibody is available for research use only.

• Storage Conditions:

  Store the CDK4 antibody at -20°C.

• Formulation:

  In PBS with 50% glycerol and 0.03% ProClin 300

• Applications Notes:

  The stated application concentrations are suggested starting points. Titration of the CDK4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of human 1) HeLa and 2) K562 cell lysates using CDK4 antibody at 1:500. Predicted molecular weight ~34 kDa.