LARGE Antibody

• Description:

  Bifunctional glycosyltransferase with both xylosyltransferase and beta-1,3-glucuronyltransferase activities involved in the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine- beta-4- (phosphate-6-) mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1) . Phosphorylated O- mannosyl trisaccharid is required for binding laminin G-like domain-containing extracellular proteins with high affinity and plays a key role in skeletal muscle function and regeneration. LARGE elongates the glucuronyl-beta-1,4-xylose-beta disaccharide primer structure initiated by B3GNT1/B4GAT1 by adding repeating units [-3-Xylose-alpha-1,3-GlcA-beta-1-] to produce a heteropolysaccharide.

• Specifications:

  Western blot: 1:1000-2000

• Gene ID:

  9215

• UniProt:

  O95461

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 365-398 from the human protein was used as the immunogen for the LARGE antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  WB

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the LARGE antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This LARGE antibody is available for research use only.

• Storage Conditions:

  Aliquot the LARGE antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the LARGE antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• Prediction Reactivity:

  Chicken, Mouse

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of Jurkat cell lysate with LARGE antibody at 1:2000. Predicted molecular weight: 88 kDa.