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Caspase-4 Antibody (large subunit)

CAT: 0800-R30563Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R30563Size:100 µg
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Description
Caspase-4 is an enzyme that proteolytically cleaves other proteins at an aspartic acid residue, and belongs to a family of cysteine proteases called caspases. The CASP4 gene is mapped to a P1 clone containing the ICE gene, which is located at chromosome 11q22.2-q22.3. It contains 8 coding exons. The function of Caspase-4 is not fully known, but it is believed to be an inflammatory caspase, along with CASP1, CASP5 and the murine homolog CASP11, with a role in the immune system.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL
UniProt
P49662
Host
Rabbit
Reactivity
Human
Immunogen
Amino acids 104-124 (DALKLCPHEEFLRLCKERAEE-human) were used as the immunogen for this Caspase-4 antibody. This sequence is from the large subunit.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
After reconstitution, the Caspase-4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Caspase-4 antibody is available for research use only.
Storage Conditions
After reconstitution, the Caspase-4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting points. Titration of the Caspase-4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of human 1) MCF-7, 2) HeLa, 3) Jurkat, 4) CCRF-CEM, 5) SW620 cell lysate with Caspase-4 antibody. Expected molecular weight ~43 kDa (precursor), ~30 kDa (pro domain + large subunit), ~20 kDa (large subunit) .

UniProtKB · P49662

Caspase-4

CASP4_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P49662
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
CASP4
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
CASP-4
EC number
3.4.22.57
Processing
Precursor
Secondary accessions
A2NHL8, A2NHL9, A2NHM0, B3KPZ9, B4DJH5, B4E2D2, O95601, Q7KYX7, Q9UG96
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

AcetylationPhosphoproteinZymogen

Coding sequence diversity

Alternative splicing

Cellular component

CytoplasmEndoplasmic reticulumInflammasomeMembraneMitochondrionSecreted

Molecular function

HydrolaseProteaseThiol protease

Biological process

ImmunityInflammatory responseInnate immunityNecrosis

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