Dnmt3a Antibody

• Description:

  Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity.

• Specifications:

  Western blot: 1:1000

• UniProt:

  Q9Y6K1

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This Dnmt3a antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 463-497 amino acids from the central region of human Dnmt3a.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  WB, ELISA

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the Dnmt3a antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This Dnmt3a antibody is available for research use only.

• Storage Conditions:

  Aliquot the Dnmt3a antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the Dnmt3a antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• Prediction Reactivity:

  Chicken, Mouse, Rat

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot analysis of lysate from (1) HeLa cell line and (2) human skeletal muscle tissue using Dnmt3a antibody at 1:1000. Predicted molecular weight: 100-130 kDa