Dnmt3l Antibody

• Description:

  DNA (cytosine-5) -methyltransferase 3-like is an enzyme that in humans is encoded by the DNMT3L gene. CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein that is a catalytically inactive regulatory factor of DNA methyltransferases. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

• Specifications:

  Western blot: 1-2 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

• UniProt:

  Q9CWR8

• Host:

  Rabbit

• Reactivity:

  Mouse, Rat

• Immunogen:

  An E. coli-derived mouse protein (amino acids A43-L421) was used as the immunogen for the Dnmt3l antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, FACS, Direct ELISA

• Purity:

  Affinity purified

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose

• Reconstitution:

  After reconstitution, the Dnmt3l antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This Dnmt3l antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the Dnmt3l antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the Dnmt3l antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Image Legend:

  Flow cytometry testing of mouse ANA-1 cells with Dnmt3l antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= Dnmt3l antibody.