Dnmt1 Antibody

• Description:

  DNA (cytosine-5) -methyltransferase 1 is an enzyme that in humans is encoded by the DNMT1 gene. This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants.

• Specifications:

  Western Blot: 0.5-1 µg/mL, Immumofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

• UniProt:

  P26358

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A recombinant human partial protein corresponding to amino acids D22-N126 was used as the immunogen for the Dnmt1 antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, IF, FACS, ELISA, IHC-P

• Purity:

  Antigen affinity purified

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose

• Reconstitution:

  After reconstitution, the Dnmt1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This Dnmt1 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the Dnmt1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the Dnmt1 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Nuclear

• Image Legend:

  Immunofluorescent staining of FFPE human U-2 OS cells with Dnmt1 antibody (green) and Alpha Tubulin mAb (red) . HIER: steam section in pH6 citrate buffer for 20 min.