Caspase-3 Antibody

• Description:

  Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly (ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.

• Specifications:

  Western blot: 1:2000

• UniProt:

  P42574

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Purified His-tagged protein was used to produced this monoclonal Caspase-3 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Clone:

  1262CT521.280.101

• Applications:

  WB, ELISA

• Purity:

  Purified

• Format:

  Purified

• Buffer:

  In 1X PBS pH 7.4 with 0.09% sodium azide

• Reconstitution:

  Aliquot the Caspase-3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This Caspase-3 antibody is available for research use only.

• Storage Conditions:

  Aliquot the Caspase-3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the Caspase-3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Caspase-3 antibody western blot analysis in Jurkat, Ramos lysate