Cdk4 Antibody

• Description:

  Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G (1) /S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G (1) phase. Hypophosphorylates RB1 in early G (1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (By similarity) .

• Specifications:

  IHC (Paraffin) : 1:25, Immunofluorescence: 1:25

• UniProt:

  P35426

• Host:

  Rabbit

• Reactivity:

  Human, Rat

• Immunogen:

  A portion of amino acids 272-303 from the rat protein was used as the immunogen for this Cdk4 antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  IHC, IF, ELISA

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the Cdk4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This Cdk4 antibody is available for research use only.

• Storage Conditions:

  Aliquot the Cdk4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the Cdk4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Location:

  Nuclear and cytoplasmic

• Image Legend:

  IHC testing of paraffin-embedded rat small intestine using Cdk4 antibody at 1:25.