B-RAF Antibody

CAT: 0800-F50793-0.08MLSize: 0.08 mLDry Ice: NoHazardous: No
CAT#:0800-F50793-0.08MLSize:0.08 mL
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PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
B-RAF, a member of the RAF subfamily of Ser/Thr protein kinases, is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It may play a role in the postsynaptic responses of hippocampal neurons. This cytoplasmic protein is expressed in brain and testis. Defects in BRAF are involved in a wide range of cancers including lung cancer and non-Hodgkin lymphoma (NHL) . This protein contains 1 zinc-dependent phorbol-ester and DAG binding domain.
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50
UniProt
P15056
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 379-408 from the human protein was used as the immunogen for this B-RAF antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
IHC, WB, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the B-RAF antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This B-RAF antibody is available for research use only.
Storage Conditions
Aliquot the B-RAF antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the B-RAF antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Mouse
Image Legend
Western blot analysis B-RAF antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BRAF gene (2) . Predicted size 85-95 kDa

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