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C-Raf Antibody / Raf1

CAT: 0800-F54689-0.08MLSize: 0.08 mLDry Ice: NoHazardous: No
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CAT#:0800-F54689-0.08MLSize:0.08 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3 (By similarity) .
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1:25, Flow cytometry: 1:25 (1x10e6 cells), Western blot: 1:500-1:2000
UniProt
Q99N57
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
A portion of amino acids 86-113 from the mouse protein was used as the immunogen for the c-Raf antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
IHC-P, FACS, WB
Purity
Antigen affinity purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the c-Raf antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This c-Raf antibody is available for research use only.
Storage Conditions
Aliquot the c-Raf antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
The stated application concentrations are suggested starting points. Titration of the c-Raf antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Cytoplasmic, nuclear
Image Legend
IHC testing of FFPE mouse skeletal muscle tissue with c-Raf antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

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