C-Raf Antibody / Raf1

RAF proto-oncogene serine/threonine-protein kinase, also known as proto-oncogene c-RAF, c-Raf or Raf-1, is an enzyme that in humans is encoded by the RAF1 gene. This gene is the cellular homolog of viral raf gene (v-raf) . The encoded protein is a MAP kinase kinasekinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.

9007-83-4

Western Blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/10^6 cells

P04049

Human, Mouse, Rat

A recombinant human protein corresponding to amino acids Y364-Q451 was used as the immunogen for the c-Raf antibody.

WB, FACS

Antigen affinity

Antigen affinity purified

Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide

After reconstitution, the c-Raf antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

This c-Raf antibody is available for research use only.

After reconstitution, the c-Raf antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the c-Raf antibody should be determined by the researcher.