CDK4 Antibody

• Description:

  CDK4 is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16 (INK4a) . This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb) . Mutations in this gene as well as in its related proteins including D-type cyclins, p16 (INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of the gene have been reported.

• Specifications:

  Immunofluorescence: 1:10-1:50, Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Flow Cytometry: 1:10-1:50

• UniProt:

  P11802

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 273-305 from the human protein was used as the immunogen for this CDK4 antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  FACS, IHC, WB, IF, ELISA

• Purity:

  Purified

• Format:

  Purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the CDK4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This CDK4 antibody is available for research use only.

• Storage Conditions:

  Aliquot the CDK4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the CDK4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Location:

  Nuclear and cytoplasmic

• Image Legend:

  Western blot analysis of CDK4 antibody and HL-60 cell lysate. Predicted molecular weight ~34 kDa.