CDK2 Antibody

• Description:

  CDK2 is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and is regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B) . Its activity is also regulated by its protein phosphorylation.

• Specifications:

  Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50

• UniProt:

  P24941

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 1-30 from the human protein was used as the immunogen for this CDK2 antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  IHC, WB, ELISA

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the CDK2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This CDK2 antibody is available for research use only.

• Storage Conditions:

  Aliquot the CDK2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the CDK2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• Prediction Reactivity:

  Mouse, Rat, Bovine, Hamster, Xenopus

• CAS Number:

  9007-83-4

• Image Legend:

  Cdk2 antibody western blot analysis in 293, HeLa lysate. Predicted molecular weight ~33 kDa.