Cdk2 Antibody

• Description:

  CDK2, Cyclin-Dependent Kinase2, is also known as P33. The CDK2 protein is highly homologous to p34 (CDC2) kinase and more significantly homologous to Xenopus Eg1 kinase, suggesting that CDK2 is the human homolog of Eg1. The CDK2 gene is mapped to 12q13, the same region to which the CDK4 gene maps. Human cyclin A binds independently to 2 kinases, p34 (cdc2) or p33. In adenovirus-transformed cells, the viral E1A oncoprotein seems to associate with p33/cyclin A but not with p34 (cdc2) /cyclin A. The gene for p33 shares 65% sequence identity with p34 (cdc2) . P33 (cdk2) plays a unique role in cell cycle regulation of vertebrate cells.

• Specifications:

  Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL

• UniProt:

  P24941

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Amino acids 81-298 of human Cdk2 were used as the immunogen for the Cdk2 antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, IHC-P, IF

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose

• Reconstitution:

  After reconstitution, the Cdk2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This Cdk2 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the Cdk2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the Cdk2 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Nuclear and cytoplasmic

• Image Legend:

  Immunofluorescent staining of FFPE human A549 cells with Cdk2 antibody (green) and Beta Tubulin mAb (red) . HIER: steam section in pH6 citrate buffer for 20 min.