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HNF4A Antibody

CAT: 0800-F48198-0.08MLSize: 0.08 mLDry Ice: NoHazardous: No
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CAT#:0800-F48198-0.08MLSize:0.08 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1-antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1-alpha. May be essential for development of the liver, kidney and intestine. [UniProt]
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50, Immunofluorescence: 1:10-1:50, Flow Cytometry: 1:10-1:50
UniProt
P41235
Host
Rabbit
Reactivity
Human, Rat
Immunogen
A portion of amino acids 281-312 from the human protein was used as the immunogen for this HNF4A antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
IF, FACS, WB, IHC, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the HNF4A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This HNF4A antibody is available for research use only.
Storage Conditions
Aliquot the HNF4A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the HNF4A antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Mouse
Location
Predominantly nuclear, present in cytoplasm in cells with high HNF4A expression
Image Legend
HNF4A antibody western blot analysis in 1) 293, 2) HepG2 cell line and 3) rat liver tissue lysate. Predicted molecular weight ~50 kDa.

UniProtKB · P41235

Hepatocyte nuclear factor 4-alpha

HNF4A_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P41235
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
HNF4A
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
HNF-4-alpha
EC number
—
Processing
—
Secondary accessions
A5JW41, B2RPP8, O00659, O00723, Q14540, Q5QPB8, Q6B4V5, Q6B4V6, Q6B4V7, Q92653, Q92654, Q92655, Q99864, Q9NQH0
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

AcetylationIsopeptide bondPhosphoproteinUbl conjugation

Molecular function

ActivatorDNA-bindingReceptorRepressor

Coding sequence diversity

Alternative promoter usageAlternative splicing

Biological process

Biological rhythmsTranscriptionTranscription regulation

Disease

Diabetes mellitusDisease variant

Ligand

Metal-bindingZinc

Cellular component

Nucleus

Domain

Zinc-finger

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