CD8 Antibody

CAT: 0800-F43566-0.4MLSize: 0.4 mLDry Ice: NoHazardous: No
CAT#:0800-F43566-0.4MLSize:0.4 mL
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PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a co-receptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional co-receptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50, Flow Cytometry: 1:10-1:50
UniProt
P01732
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 150-180 from the human protein was used as the immunogen for this CD8 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, IHC, FACS, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the CD8 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This CD8 antibody is available for research use only.
Storage Conditions
Aliquot the CD8 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the CD8 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Flow cytometric analysis of NCI-H292 cells using CD8 antibody (bottom histogram) compared to a negative control (top histogram) . FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

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