Dnmt3a Antibody

• Description :

  CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. Dnmt3a is a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. [Wiki]

• Specifications :

  Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50

• UniProt :

  Q9Y6K1

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  A portion of amino acids 457-486 from the human protein was used as the immunogen for this Dnmt3a antibody.

• Clonality :

  Polyclonal

• Isotype :

  Ig

• Applications :

  WB, IHC, ELISA

• Purity :

  Antigen affinity

• Format :

  Antigen affinity purified

• Buffer :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution :

  Aliquot the Dnmt3a antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations :

  This Dnmt3a antibody is available for research use only.

• Storage Conditions :

  Aliquot the Dnmt3a antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes :

  Titration of the Dnmt3a antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• Prediction Reactivity :

  Mouse, Rat, Chicken

• CAS Number :

  9007-83-4

• Image Legend :

  Western blot analysis of Dnmt3A antibody and Jurkat lysate. Predicted molecular weight: 100-130 kDa