Dnmt3a Antibody

• Description:

  CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. Dnmt3a is a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated.

• Specifications:

  Western blot: 1:1000

• UniProt:

  Q9Y6K1

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 723-752 from the human protein was used as the immunogen for this Dnmt3a antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  WB, ELISA

• Purity:

  Purified

• Format:

  Purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the Dnmt3a antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This Dnmt3a antibody is available for research use only.

• Storage Conditions:

  Aliquot the Dnmt3a antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the Dnmt3a antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• Prediction Reactivity:

  Mouse, Rat

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot analysis of Dnmt3a antibody and HepG2 lysate. Predicted molecular weight: 100-130 kDa