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LAG3 Detection Set (Risk Free)

CAT: 0223-RF16080Size: 1 SetDry Ice: NoHazardous: No
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CAT#:0223-RF16080Size:1 Set
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
The lymphocyte activation gene-3 (LAG3) is a member of the immunoglobulin superfamily and binds MHC class II with high affinity (1), negatively regulating T-cell function and homeostasis (2) . It is expressed in B, T, and NK cells, monocytes, and dendritic cells (3), and acts to regulate T cell expansion (4) . LAG3 is also an important immune checkpoint protein, with anti-LAG3 antibodies activating T effector cells and affecting regulatory T cell functions. Furthermore LAG3 appears to act in a synergistic fashion with PD-1/PD-L1, suggesting that a dual antibody approach may prove useful in cancer immunotherapy. ProSci's Risk-FreeTM antibodies are mouse monoclonal antibodies made to improve in vivo studies. Unlike antibodies developed using proteins made in yeast or bacteria, Risk-FreeTM antibodies are developed with antigens expressed in mammalian cell lines, giving the most native post-translational modifications to the protein. Validated for flow cytometry and ELISA Rigorously tested for the following applications: Immunoblot Immunohistochemistry Immunocytochemistry Immunofluorescence Multiple antibodies per target allowing the user to choose the best antibody for their application Available individually or as a set Risk-FreeTM means they are guaranteed to work for their approved applications
Host
Mouse
Reactivity
Human
Immunogen
LAG3 antibodies were raised against the extracellular domain of human LAG3.
Clonality
Monoclonal
Conjugation
Unconjugated
Type
Detection Sets
Purification
Antibodies are supplied as protein A purified IgG1 and IgG2a.
Positive Control
Cat. No. RF10001-03 - Human Spleen Tissue Lysate
Concentration
Antibody 1 mg/mL
Buffer
PBS containing 0.02% sodium azide.
Shipping Conditions
Blue Ice
Storage Conditions
Antibodies can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Fragment
IgG1, IgG2a
Background Reference 01
Huard B, Tournier M, Hercend T, et al. Lymphocyte-activation gene 3/major histocompatibility complex class II interaction modulates the antigenic response of CD4+ T lymphocytes. Eur J Immunol 1994; 24:3216–21.
Background Reference 02
Triebel F. LAG-3: a regulator of T-cell and DC responses and its use in therapeutic vaccination. Trends Immunol 2003; 24:619–22.
Background Reference 03
Workman CJ, Wang Y, El Kasmi KC, et al. LAG-3 regulates plasmacytoid dendritic cell homeostasis. J Immunol. 2009; 182:1885–91.
Background Reference 04
Workman CJ, Vignali DA. The CD4-related molecule, LAG-3 (CD223), regulates the expansion of activated T cells. Eur J Immunol 2003; 33:970–9.
Tested Applications
ELISA, WB, ICC, IHC-P, Flow, IF
Physical Properties
Liquid

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