CTLA4 Detection Set (Risk Free)

• Background:

  The cytotoxic T-lymphocyte-associated protein 4 (CTLA-4), also known as CD152, is a member of the immunoglobulin superfamily that is expressed by activated T cells and transmits an inhibitory signal to T cells (1,2) . Both it and the homologous T-cell co-stimulatory protein CD28 bind to CD80 (B7-H1) and CD86 (B7-H2) on antigen-presenting cells (APCs) (3) . Mutations in the CTLA-4 gene have been implicated in multiple autoimmune diseases (4) . CTLA-4 also functions as an immune checkpoint protein, and anti- CTLA-4 antibodies have been successfully used in the treatment of cancer (5) . ProSci's Risk-FreeTM antibodies are mouse monoclonal antibodies made to improve in vivo studies. Unlike antibodies developed using proteins made in yeast or bacteria, Risk-FreeTM antibodies are developed with antigens expressed in mammalian cell lines, giving the most native post-translational modifications to the protein. Validated for flow cytometry and ELISA Rigorously tested for the following applications: Immunoblot Immunohistochemistry Immunocytochemistry Immunofluorescence Multiple antibodies per target allowing the user to choose the best antibody for their application Available individually or as a set Risk-FreeTM means they are guaranteed to work for their approved applications

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Mouse monoclonal CTLA-4 antibodies were raised against the extracellular domain of human CTLA-4.

• Clonality:

  Monoclonal

• Conjugation:

  Unconjugated

• Type:

  Detection Sets

• Purification:

  Antibodies are supplied as protein A purified IgG1.

• Positive Control:

  Cat. No. RF10001-04 - Human Tonsil Tissue Lysate

• Concentration:

  Antibody 1 mg/mL

• Buffer:

  PBS containing 0.02% sodium azide.

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Antibodies can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG1

• Positive Control 2:

  Cat. No. RF10001-05 - Lymph Node Tissue Lysate

• Background Reference 01:

  Brunet JF, Denizot F, Luciani MF, et al. A new member of the immunoglobulin superfamily--CTLA-4 . Nature 1987; 328:267–70.

• Background Reference 02:

  Walunas TL, Lenschow DJ, Bakker CY, et al. CTLA-4 can function as a negative regulator of T cell activation. Immunity 1994; 1:405–13.

• Background Reference 03:

  Harding FA, McArthur JG, Gross JA, et al. CD28-mediated signalling co-stimulates murine T cells and prevents induction of anergy in T-cell clones. Nature 1992; 356:607–9.

• Background Reference 04:

  Romo-Tena J, Gomez-Martin D, and Alcocer-Verela J. CTLA-4 and autoimmunity: new insights into the dual regulator of tolerance. Autoimmun. Rev. 2013; 12:117.

• Tested Applications:

  ELISA, WB, ICC, IHC-P, Flow, IF

• Physical Properties:

  Liquid