CD80 Detection Set (Risk Free)

• Background:

  CD80, also known as B7-1, is a type I membrane protein that is a member of the immunoglobulin superfamily. Like the related protein CD86, this protein is expressed by antigen-presenting cells, and is the ligand for two proteins at the cell surface of T cells, CD28 and the cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) . Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell and induces T-cell proliferation and cytokine production. CTLA-4 binding negatively regulates T-cell activation and diminishes the immune response (1) . Blocking the CTLA-4-CD80/CD86 interaction has been shown to enhance T-cell functions in acute lymphoblastomic leukemia (ALL), suggesting that this pathway may be an attractive target for future cancer immunotherapy (2) . ProSci's Risk-FreeTM antibodies are mouse monoclonal antibodies made to improve in vivo studies. Unlike antibodies developed using proteins made in yeast or bacteria, Risk-FreeTM antibodies are developed with antigens expressed in mammalian cell lines, giving the most native post-translational modifications to the protein. Validated for flow cytometry and ELISA Rigorously tested for the following applications: Immunoblot Immunohistochemistry Immunocytochemistry Immunofluorescence Multiple antibodies per target allowing the user to choose the best antibody for their application Available individually or as a set Risk-FreeTM means they are guaranteed to work for their approved applications

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  CD80 antibodies were raised against the extracellular domain of human CD80.

• Clonality:

  Monoclonal

• Conjugation:

  Unconjugated

• Type:

  Detection Sets

• Purification:

  Antibodies are supplied as protein A purified IgG1 and IgG2b.

• Positive Control:

  Cat. No. RF10001-06 - Raji Lysate

• Concentration:

  Antibody 1 mg/mL

• Buffer:

  PBS containing 0.02% sodium azide.

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Antibodies can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG1, IgG2b

• Positive Control 2:

  Cat. No. RF10001-04 - Human Tonsil Tissue Lysate

• Background Reference 01:

  Lane P. Regulation of T and B cell responses by modulating interactions between CD28/CTLA-4 and their ligands, CD80 and CD86. Ann NY Acad Sci 1997; 815:392-400.

• Background Reference 02:

  Feucht J, Kayser S, Gorodezki D, et al. T-cell responses against CD19+ pediatric acute lymphoblastic leukemia mediated by bispecific T-cell engager (BiTE) are regulated contrarily by PD-L1 and CD80/CD86 on leukemic blasts. Oncotarget 2016; 7:76902-19.

• Tested Applications:

  ELISA, WB, ICC, IHC-P, Flow, IF

• Physical Properties:

  Liquid